OrangerV1, Main, Exploration, bibRecord, 001319

Fruit load modulates flowering-related gene expression in buds of alternate-bearing ‘Moncada’ mandarin

Identifieur interne : 001319 ( Main/Exploration ); précédent : 001318; suivant : 001320

Fruit load modulates flowering-related gene expression in buds of alternate-bearing ‘Moncada’ mandarin

Auteurs : Natalia Mu Oz-Fambuena [Espagne] ; Carlos Mesejo [Espagne] ; M. Carmen González-Mas [Espagne] ; Eduardo Primo-Millo [Espagne] ; Manuel Agustí [Espagne] ; Domingo J. Iglesias [Espagne]

Source :

Annals of Botany [ 0305-7364 ] ; 2012.

RBID : PMC:3478051

Abstract

Background and Aims

Gene determination of flowering is the result of complex interactions involving both promoters and inhibitors. In this study, the expression of flowering-related genes at the meristem level in alternate-bearing citrus trees is analysed, together with the interplay between buds and leaves in the determination of flowering.

Methods

First defruiting experiments were performed to manipulate blossoming intensity in ‘Moncada’ mandarin, Citrus clementina. Further defoliation was performed to elucidate the role leaves play in the flowering process. In both cases, the activity of flowering-related genes was investigated at the flower induction (November) and differentiation (February) stages.

Key Results

Study of the expression pattern of flowering-genes in buds from on (fully loaded) and off (without fruits) trees revealed that homologues of FLOWERING LOCUS T (CiFT), TWIN SISTER OF FT (TSF), APETALA1 (CsAP1) and LEAFY (CsLFY) were negatively affected by fruit load. CiFT and TSF activities showed a marked increase in buds from off trees through the study period (ten-fold in November). By contrast, expression of the homologues of the flowering inhibitors of TERMINAL FLOWER 1 (CsTFL), TERMINAL FLOWER 2 (TFL2) and FLOWERING LOCUS C (FLC) was generally lower in off trees. Regarding floral identity genes, the increase in CsAP1 expression in off trees was much greater in buds than in leaves, and significant variations in CsLFY expression (approx. 20 %) were found only in February. Defoliation experiments further revealed that the absence of leaves completely abolished blossoming and severely affected the expression of most of the flowering-related genes, particularly decreasing the activity of floral promoters and of CsAP1 at the induction stage.

Conclusions

These results suggest that the presence of fruit affects flowering by greatly altering gene-expression not only at the leaf but also at the meristem level. Although leaves are required for flowering to occur, their absence strongly affects the activity of floral promoters and identity genes.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478051

DOI: 10.1093/aob/mcs190
PubMed: 22915579
PubMed Central: 3478051

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><sec><title>Background and Aims</title>
<p>Gene determination of flowering is the result of complex interactions involving both promoters and inhibitors. In this study, the expression of flowering-related genes at the meristem level in alternate-bearing citrus trees is analysed, together with the interplay between buds and leaves in the determination of flowering.</p>
</sec>
<sec><title>Methods</title>
<p>First defruiting experiments were performed to manipulate blossoming intensity in ‘Moncada’ mandarin, <italic>Citrus clementina</italic>
. Further defoliation was performed to elucidate the role leaves play in the flowering process. In both cases, the activity of flowering-related genes was investigated at the flower induction (November) and differentiation (February) stages.</p>
</sec>
<sec><title>Key Results</title>
<p>Study of the expression pattern of flowering-genes in buds from <italic>on</italic>
 (fully loaded) and <italic>off</italic>
 (without fruits) trees revealed that homologues of <italic>FLOWERING LOCUS T</italic>
 (<italic>CiFT</italic>
), <italic>TWIN SISTER OF FT</italic>
 (<italic>TSF</italic>
), <italic>APETALA1</italic>
 (<italic>CsAP1</italic>
) and <italic>LEAFY</italic>
 (<italic>CsLFY</italic>
) were negatively affected by fruit load. <italic>CiFT</italic>
 and <italic>TSF</italic>
 activities showed a marked increase in buds from <italic>off</italic>
 trees through the study period (ten-fold in November). By contrast, expression of the homologues of the flowering inhibitors of <italic>TERMINAL FLOWER 1</italic>
 (<italic>CsTFL</italic>
), <italic>TERMINAL FLOWER 2</italic>
 (<italic>TFL2</italic>
) and <italic>FLOWERING LOCUS C</italic>
 (<italic>FLC</italic>
) was generally lower in <italic>off</italic>
 trees. Regarding floral identity genes, the increase in <italic>CsAP1</italic>
 expression in <italic>off</italic>
 trees was much greater in buds than in leaves, and significant variations in <italic>CsLFY</italic>
 expression (approx. 20 %) were found only in February. Defoliation experiments further revealed that the absence of leaves completely abolished blossoming and severely affected the expression of most of the flowering-related genes, particularly decreasing the activity of floral promoters and of <italic>CsAP1</italic>
 at the induction stage.</p>
</sec>
<sec><title>Conclusions</title>
<p>These results suggest that the presence of fruit affects flowering by greatly altering gene-expression not only at the leaf but also at the meristem level. Although leaves are required for flowering to occur, their absence strongly affects the activity of floral promoters and identity genes.</p>
</sec>
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<name sortKey="Agusti, Manuel" sort="Agusti, Manuel" uniqKey="Agusti M" first="Manuel" last="Agustí">Manuel Agustí</name>
<name sortKey="Gonzalez Mas, M Carmen" sort="Gonzalez Mas, M Carmen" uniqKey="Gonzalez Mas M" first="M. Carmen" last="González-Mas">M. Carmen González-Mas</name>
<name sortKey="Iglesias, Domingo J" sort="Iglesias, Domingo J" uniqKey="Iglesias D" first="Domingo J." last="Iglesias">Domingo J. Iglesias</name>
<name sortKey="Mesejo, Carlos" sort="Mesejo, Carlos" uniqKey="Mesejo C" first="Carlos" last="Mesejo">Carlos Mesejo</name>
<name sortKey="Primo Millo, Eduardo" sort="Primo Millo, Eduardo" uniqKey="Primo Millo E" first="Eduardo" last="Primo-Millo">Eduardo Primo-Millo</name>
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Fruit load modulates flowering-related gene expression in buds of alternate-bearing ‘Moncada’ mandarin

Fruit load modulates flowering-related gene expression in buds of alternate-bearing ‘Moncada’ mandarin

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Abstract

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